FUS/TLS Recombinant Rabbit Monoclonal Antibody [JJ09-31]

About this product

EWS and FUS/TLS are nuclear RNA-binding proteins. As a result of chromosome translocation, the EWS gene is fused to a variety of transcription factors, including ATF-1, in human neoplasias. In the Ewing family of tumors, the N-terminal domain of EWS is fused to the DNA-binding domain of various Ets transcription factors, including Fli-1, ETV1 and FEV. The EWS/Fli-1 chimeric protein acts as a more potent transcriptional activator than Fli-1 and can promote cell transformation. In human myxoid liposarcomas and myeloid leukemias, chromosomal translocation results in the fusion of the N-terminal region of FUS/TLS with the open reading frame of CHOP. In normal cells, FUS/TLS binds to the DNA-binding domains of nuclear steroid receptors and is also present in subpopulations of TFIID complexes, indicating a potential role for FUS/TLS in the processing of primary transcripts that are generated in response to hormone-induced transcription.

Specifications

Catalog number

ET1701-86

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

75 kDa DNA pairing protein antibody 75 kDa DNA-pairing protein antibody ALS6 antibody Amyotrophic lateral sclerosis 6 antibody fus antibody FUS CHOP antibody Fus like protein antibody FUS_HUMAN antibody FUS1 antibody Fused in sarcoma antibody Fusion (involved in t (12;16) in malignant liposarcoma) antibody Fusion derived from t (12;16) malignant liposarcoma antibody Fusion gene in myxoid liposarcoma antibody Heterogeneous nuclear ribonucleoprotein P2 antibody hnRNP P2 antibody hnRNPP2 antibody Oncogene FUS antibody Oncogene TLS antibody POMp75 antibody RNA binding protein FUS antibody RNA-binding protein FUS antibody TLS antibody TLS CHOP antibody Translocated in liposarcoma antibody Translocated in liposarcoma protein antibody

Abbreviation

FUS

Swiss Prot

P35637 Human, P56959 Mouse

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human aa 1-46 / 526.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC, IF-Cell

Positive Control

K562 cell lysates, HepG2, NIH/3T3, human tonsil tissue, human colon carcinoma tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:5,000 ;IHC-P: 1:50-1:200 ;FC: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes