Bmi1 Recombinant Rabbit Monoclonal Antibody [JJ093-3]

About this product

BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. Bmi1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Recently it has been reported BMI1 is rapidly recruited to sites of DNA damage and it sustains for over 8h. Loss of BMI1 leads to radiation sensitive and impaired repair of DNA double-strand breaks by homologous recombination. Bmi1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells.[5][6] However, it is less important for the generation of differentiated progeny. Given that phenotypic changes in Bmi1 knockout mice are numerous and that Bmi1 has very broad tissue distribution, it is possible that it regulates the self-renewal of other types of somatic stem cells. Bmi1 is also thought to inhibit ageing in neurons through the suppression of p53. The Bmi-1 expression interacts with several signaling containing Wnt, Akt, Notch, Hedgehog and receptor tyrosine kinase (RTK) pathway. In Ewing sarcoma family of tumors (ESFT), the knockdown of BMI-1 gene would greatly influence the Notch and Wnt signaling pathway which are important for ESFT formation and development. Bmi-1 was shown to mediate the effect of Hedgehog signaling pathway on mammary stem cell proliferation.[Bmi-1 also regulates multiple downstream factors or genes. It represses p19Arf and p16Ink4a. Bmi-1-/- neural stem cells and HSCs have high expression level of p19Arf and p16Ink4a which diminished the proliferation rate.Bmi-1 is also indicated as a key factor in controlling Th2 cell differentiation and development by stabilizing GATA transcription factors.

Specifications

Catalog number

ET1701-89

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

B lymphoma Mo MLV insertion region (mouse) antibody B lymphoma Mo MLV insertion region 1 homolog antibody Bmi 1 antibody BMI1 antibody BMI1 polycomb ring finger oncogene antibody BMI1_HUMAN antibody Flvi 2/bmi 1 antibody FLVI2/BMI1 antibody MGC12685 antibody Murine leukemia viral (bmi 1) oncogene homolog antibody Oncogene BMI 1 antibody PCGF 4 antibody PCGF4 antibody Polycomb complex protein BMI 1 antibody Polycomb complex protein BMI-1 antibody Polycomb group protein Bmi1 antibody Polycomb group ring finger 4 antibody Polycomb group RING finger protein 4 antibody RING finger protein 51 antibody RNF 51 antibody RNF51 antibody

Abbreviation

BMI1

Swiss Prot

P35226 Human,

Cellular Locus

Nucleus, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Rat

Immunogen

Synthetic peptide within Human Bmi1 aa 282-326 / 326.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

HeLa cell lysate, A549 cell lysate, PC-12 cell lysate, Rat brain tissue lysate, human kidney tissue, rat kidney tissue, Hela, A549, SW480.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 37 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes