Apolipoprotein A1 Recombinant Rabbit Monoclonal Antibody [JF0548]

About this product

Apolipoprotein AI (Apo-AI) is a protein that in humans is encoded by the APOA1 gene.As the major component of HDL particles, it has a specific role in lipid metabolism.Apolipoprotein AI is the major protein component of high density lipoprotein (HDL) particles in plasma.[10] Chylomicrons secreted from the intestinal enterocyte also contain apo-AI, but it is quickly transferred to HDL in the bloodstream.[11] The protein, as a component of HDL particles, enables efflux of fat molecules by accepting fats from within cells (including macrophages within the walls of arteries which have become overloaded with ingested fats from oxidized LDL particles) for transport (in the water outside cells) elsewhere, including back to LDL particles or to the liver for excretion. It is a cofactor for lecithin—cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Apolipoprotein AI has also been isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect.[12] Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.Apo-AI is often used as a biomarker for prediction of cardiovascular diseases. The ratio apoB-100/apoA1 (i.e. LDL & larger particles vs. HDL particles), NMR measured lipoprotein (low density lipoprotein (LDL)/ (HDL) particle ratios even more so, has always had a stronger correlation with myocardial infarction event rates than older methods of measuring lipid transport in the water outside cells. Apo-AI is routinely measured using immunoassays such as ELISA or nephelometry.

Specifications

Catalog number

ET1702-23

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Apo-AI antibody ApoA I antibody ApoA-I antibody APOA1 antibody APOA1_HUMAN antibody Apolipoprotein A-I (1-242) antibody Apolipoprotein A1 antibody Apolipoprotein AI antibody Brp14 antibody Ltw1 antibody Lvtw1 antibody Sep1 antibody Sep2 antibody

Abbreviation

Apo-AI, ApoA-I, APOA1

Swiss Prot

P02647 Human

Cellular Locus

Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within human Apolipoprotein A1 aa 1-50.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

Human plasma lysates, HepG2, Hela, human liver tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 31 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes