Progesterone Receptor Recombinant Rabbit Monoclonal Antibody [JF0549]

About this product

PR, a protein with 946 amino acids, is a ligand-activated transcription factor member of the steroid receptor super family of nuclear receptors. The functional structure is similar to that of estrogen receptor (ER), with considerable sequence homology in the DNA-binding central domain. PR is predominantly expressed in tumours of female sex steroid responsive tissues such as the mammary gland, endometrium and the ovary. About half of the breast carcinomas are ER+/PR+. A small fraction (<5%) is ER-/PR+. About half of the non-mucinous ovarian carcinomas are also PR+. From other PR-expressing tumours, meningiomas, various pancreatic neoplasms such as solid-pseudopapillary tumour and endocrine tumours, and salivary gland neoplasms are worth mentioning. The ER and PR status has been used for over 20 years as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence. Up to 75% of ER+/PR+ breast carcinomas respond positively to endocrine treatment. ER+/PR- tumours are less responsive, and thus PR status adds information to ER-status. In combination the two predict benefit from endocrine therapy both in adjuvant setting and in advanced disease. In breast cancer predominance of one isoform, namely PR-B, is common. The majority of endometrial carcinomas express only one isoform. The applications of antibodies to PR are similar to those against ER, i.e. diagnosis of PR-positive tumours (often metastasis) and prediction of therapeutic response of breast carcinoma.

Specifications

Catalog number

ET1702-24

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

NR3C3 antibody Nuclear receptor subfamily 3 group C member 3 antibody PGR antibody PR antibody PRA antibody PRB antibody PRGR_HUMAN antibody Progesterone receptor antibody Progestin receptor form A antibody Progestin receptor form B antibody

Abbreviation

PR, PGR

Swiss Prot

P06401 Human

Cellular Locus

Nucleus, Cytoplasm, Mitochondrion outer membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within N-terminal human Progesterone Receptor.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

MCF-7, human breast tissue, human cervix tissue, human uterus tissue, human smooth muscle tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:500-1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

99 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes