Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882]

Catalog number ET1702-34

Price for ET1702-34

Size / count 100 µL

About this product

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Specifications

Catalog number: ET1702-34
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: Drosophila, homolog of, MADR2 antibody hMAD-2 antibody HsMAD2 antibody JV18 antibody JV18-1 antibody JV181 antibody MAD antibody MAD homolog 2 antibody MAD Related Protein 2 antibody Mad-related protein 2 antibody MADH2 antibody MADR2 antibody MGC22139 antibody MGC34440 antibody Mother against DPP homolog 2 antibody Mothers against decapentaplegic homolog 2 antibody Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody Mothers against DPP homolog 2 antibody OTTHUMP00000163489 antibody Sma and Mad related protein 2 antibody Sma- and Mad-related protein 2 MAD antibody SMAD 2 antibody SMAD family member 2 antibody SMAD, mothers against DPP homolog 2 antibody SMAD2 antibody SMAD2_HUMAN antibody
Abbreviation: MAD homolog 2, Mothers against DPP homolog 2, hMAD-2, SMAD 2, Smad2, hSMAD2
Swiss Prot: Q15796 Human, Q62432 Mouse, O70436 Rat
Cellular Locus: Nucleus, Cytoplasm.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser255 of Human Smad2 aa 229-272 / 467.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IHC-P, IP
Positive Control: HeLa cell lysate, HeLa treated with 200nM Calyculin A for 1 hour cell lysate, RAW264.7 cell lysate, mouse uterus tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 52 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882]?

The catalog number for Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882] is ET1702-34.

What sizes are available for Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882]?

Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882] is available in the following size(s): 100 µL.

Is Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882] in stock?

Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-Smad2 (S255) Recombinant Rabbit Monoclonal Antibody [JF0882] used for?

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.