MHC class I Recombinant Rabbit Monoclonal Antibody [JF10-38]

About this product

Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Specifications

Catalog number

ET1702-47

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

A 28 antibody A 9 antibody Antigen presenting molecule antibody Aw 24 antibody Aw 68 antibody CLASS I HISTOCOMPATIBILITY ANTIGEN antibody H2 K1 antibody H2K antibody HLA A antibody HLA class I histocompatibility antigen A 1 alpha chain antibody HLA class I histocompatibility antigen A 11 alpha chain antibody HLA class I histocompatibility antigen A 2 alpha chain antibody HLA class I histocompatibility antigen A 24 alpha chain antibody HLA class I histocompatibility antigen A 3 alpha chain antibody HLA class I histocompatibility antigen A 30 alpha chain antibody HLA class I histocompatibility antigen A 32 alpha chain antibody HLA class I histocompatibility antigen A 68 alpha chain antibody HLAA antibody Leukocyte antigen class I A antibody Major histocompatibility complex class I A antibody MHC class I antigen A 1 antibody MHC class I antigen A*11 antibody MHC class I antigen A*2 antibody MHC class I antigen A*24 antibody MHC class I antigen A*3 antibody MHC class I antigen A*30 antibody MHC class I antigen A*32 antibody MHC class I antigen A*68 antibody MHC class I antigen HLA A heavy chain antibody MHC class I heavy chain H2 K antibody

Abbreviation

HLA-A, HLA-B

Swiss Prot

P04439 Human, P01889 Human

Cellular Locus

Cell membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human HLA aa 263-299 / 365.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IF-Tissue

Positive Control

A431 cell lysate, HL-60 cell lysate, Jurkat cell lysate, Raji cell lysate, THP-1 cell lysate, HL-60, human colon cancer tissue, human liver cancer tissue, human tonsil tissue, human pancreas tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:1:1,000 ;FC: 1:1,000 ;IF-Tissue: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 41 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes