NQO1 Recombinant Rabbit Monoclonal Antibody [JF440-1]

About this product

NAD (P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.

Specifications

Catalog number

ET1702-50

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Azoreductase antibody Cytochrome b 5 reductase antibody DHQU antibody DIA 4 antibody DIA4 antibody Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody Diaphorase (NADH/NADPH) antibody Diaphorase 4 antibody Dioxin inducible 1 antibody DT diaphorase antibody DT-diaphorase antibody DTD antibody Menadione reductase antibody NAD (P)H dehydrogenase [quinone] 1 antibody NAD (P)H dehydrogenase quinone 1 antibody NAD (P)H menadione oxidoreductase 1 dioxin inducible antibody NAD (P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody NAD (P)H:menadione oxidoreductase 1 antibody NAD (P)H:Quinone acceptor oxidoreductase type 1 antibody NAD (P)H:quinone oxidoreductase 1 antibody NAD (P)H:quinone oxireductase antibody NMOR 1 antibody NMOR I antibody NMOR1 antibody NMORI antibody NQO 1 antibody NQO1 antibody NQO1_HUMAN antibody Phylloquinone reductase antibody QR 1 antibody QR1 antibody Quinone reductase 1 antibody

Abbreviation

DTD, QR1, NQO1

Swiss Prot

P15559 Human, Q64669 Mouse, P05982 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human NQO1 aa 1-49 / 274.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IP, FC, IHC-P

Positive Control

HCT 116 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human breast cancer tissue, MCF7.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000 ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 31 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes