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Product details

Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]

Catalog number ET1702-53

Price for ET1702-53

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Size / count 100 µL

About this product

Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the anti-apoptotic influence of Bcl-2.

Specifications

Catalog number
ET1702-53
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
Apoptosis regulator Bcl 2 antibody Apoptosis regulator Bcl-2 antibody Apoptosis regulator Bcl2 antibody AW986256 antibody B cell CLL/lymphoma 2 antibody B cell leukemia/lymphoma 2 antibody Bcl-2 antibody Bcl2 antibody BCL2_HUMAN antibody C430015F12Rik antibody D630044D05Rik antibody D830018M01Rik antibody Leukemia/lymphoma, B-cell, 2 antibody Oncogene B-cell leukemia 2 antibody PPP1R50 antibody Protein phosphatase 1, regulatory subunit 50 antibody Bcl 2 antibody
Abbreviation
BCL2
Swiss Prot
P10415 Human, P10417 Mouse, P49950 Rat
Cellular Locus
Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein within human Bcl-2 aa 1-230.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Positive Control
HeLa cell lysate, Jurkat cell lysate, MCF-7 cell lysate, HL-60 cell lysate, THP-1 cell lysate, rat spleen tissue lysate, mouse spleen tissue lysate, Hela, A549, human tonsil tissue, human colon carcinoma tissue, mouse kidney tissue, human lung carcinoma tissue, Jurkat, human B-cell lymphoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:500 ;IHC-P: 1:1,000-1:5,000 ;FC: 1:500-1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 26 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]?

The catalog number for Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8] is ET1702-53.

What sizes are available for Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8]?

Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8] is available in the following size(s): 100 µL.

Is Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8] in stock?

Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Bcl-2 Recombinant Rabbit Monoclonal Antibody [JF104-8] used for?

Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with ot

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

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