PDHA1 Recombinant Rabbit Monoclonal Antibody [JF996-0]

About this product

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial matrix enzyme complex that functions as the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The E1 enzyme of the PDH complex is made up of a heterotetramer of two α and two β subunits. The E1-α subunit (PDH-E1α) contains the E1 active site and plays a key role in the function of the PDH complex. The PDH complex is regulated by phosphorylation and dephosphorylation of PDH-E1α. The gene encoding for PDH-E1α maps to chromosome Xp22.12, and a 20bp deletion in the last exon of this gene is sufficient to cause PDH deficiency, which causes a broad range of symptoms including the development of seizures, mental retardation and spasticity, as well as intermittent episodes of lactic acidosis associated with cerebellar ataxia.

Specifications

Catalog number

ET1702-75

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ODPA_HUMAN antibody PDH antibody PDHA antibody PDHA1 antibody PDHCE1A antibody PDHE1 A type I antibody PDHE1-A type I antibody PHE1A antibody Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody Pyruvate Dehydrogenase E1 alpha antibody Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody

Abbreviation

PDHA1

Swiss Prot

P08559 Human, P35486 Mouse, P26284 Rat

Cellular Locus

Mitochondrion matrix.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human PDHA1 aa 100-145 / 390.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

Rat kidney tissue lysate, mouse stomach tissue lysate, 293T cell lysate, A431 cell lysate, mouse heart tissue lysate, Hela, HepG2, SH-SY5Y, human breast carcinoma tissue, human kidney tissue, mouse skeletal muscle tissue, human lung carcinoma tissue, mouse colon tissue, mouse stomach tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:200-1:1,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

43 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes