Neutrophil Elastase Recombinant Rabbit Monoclonal Antibody [JF098-6]

About this product

Neutrophil Elastase (NE) is a serine protease that is expressed in bone marrow precursor cells, stored in peripheral blood granulocytes, and implicated in the progression of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome and cystic fibrosis. In neutrophils, Neutrophil Elastase contributes largely to the proteolysis of phagocytosed proteins, the migration of neutrophils and the remodeling of tissues following injury. Neutrophil Elastase, which is also designated medullasin, is secreted into the extracellular matrix, where it is then capable of destroying connective tissue proteins, including elastin, proteoglycans and Type IV Collagens. Neutrophil Elastase also mediates proteolysis by cleaving proteins that are associated with the complement system, such as antithrombin and Fibrinogen. Additionally, Neutrophil Elastase functions as a potent platelet agonist, where it potentiates the aggregation, secretion and mobilization of calcium in response to cathepsin G binding to platelet surface receptors.

Specifications

Catalog number

ET1702-78

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

Bone marrow serine protease antibody ELA2 antibody ELANE antibody Elastase 2 antibody Elastase 2 neutrophil antibody Elastase neutrophil expressed antibody Elastase-2 antibody ELNE_HUMAN antibody GE antibody Granulocyte derived elastase antibody HLE antibody HNE antibody Human leukocyte elastase antibody Leukocyte elastase antibody Medullasin antibody NE antibody Neutrophil elastase antibody PMN E antibody PMN elastase antibody Polymorphonuclear elastase antibody SCN1 antibody

Abbreviation

HLE, ELANE

Swiss Prot

P08246 Human, Q3UP87 Mouse

Cellular Locus

Cytoplasmic vesicle, phagosome

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Human Neutrophil Elastase aa 1-140 / 268.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

HL-60 cell lysates, A549, Hela, HepG2, human bone marrow tissue, mouse bone tissue, human tonsil tissue, human spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:50-1:200 ;FC: 1:10-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 29 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes