ASPP2 Recombinant Rabbit Monoclonal Antibody [JF09-98]

About this product

The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.

Specifications

Catalog number

ET1702-79

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

53BP2 antibody Apoptosis stimulating of p53 protein 2 antibody Apoptosis stimulating protein of p53 2 antibody Apoptosis-stimulating of p53 protein 2 antibody ASPP2 antibody ASPP2_HUMAN antibody BBP antibody Bcl2 binding protein antibody Bcl2-binding protein antibody NY REN 51 antigen antibody p53 binding protein 2 antibody p53-binding protein 2 antibody p53BP2 antibody PPP1R13A antibody Renal carcinoma antigen NY-REN-51 antibody Tp53bp2 antibody Tumor protein p53 binding protein 2 antibody Tumor suppressor p53 binding protein 2 antibody Tumor suppressor p53-binding protein 2 antibody

Abbreviation

TP53BP2, Bbp, 53BP2, p53BP2

Swiss Prot

Q13625 Human, Q8CG79 Mouse

Cellular Locus

Nucleus, perinuclear region.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within N-terminal human ASPP2.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

Hela cell lysate, MCF-7 cell lysate, Hela, 293T, human kidney tissue, human prostate tissue, mouse testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:100-1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

150 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes