Human IgM Recombinant Rabbit Monoclonal Antibody [JF84-09]

About this product

Immunoglobulin M (IgM) is one of several isotypes of antibody (also known as immunoglobulin) that are produced by vertebrates. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. In humans and other mammals that have been studied, Plasmablasts residing in the spleen are the main source for specific IgM production. IgM interacts with several other physiological molecules: IgM can bind complement component C1 and activate the classical pathway, leading to opsonization of antigens and cytolysis; IgM binds to the polyimmunoglobulin receptor (pIgR) in a process that brings IgM to mucosal surfaces, such as the gut lumen and into breast milk. This binding depends on J chain; Two other Fc receptors that bind IgM—Fcα/µ-R and Fcµ-R -- have been detected. Fcα/µ-R, like pIgR, binds polymeric IgM and IgA. Fcα/µ-R can mediate endocytosis, and its expression in the gut suggests a role in mucosal immunity. Fcµ-R (formerly known as Toso/Faim3) binds IgM exclusively and can mediate cellular uptake of IgM-conjugated antigen.

Specifications

Catalog number

ET1702-81

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Immunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region antibody Immunoglobin heavy chain constant region mu antibody AGM1 antibody Constant region of heavy chain of IgM antibody DKFZp686I15196 antibody DKFZp686I15212 antibody FLJ00385 antibody Ig mu chain C region antibody IGHM antibody IgM heavy chain constant region antibody Immunoglobin heavy constant mu antibody Immunoglobulin mu antibody MGC104996 antibody MGC52291 antibody MU antibody VH antibody

Abbreviation

IGHM

Swiss Prot

P01871 Human

Cellular Locus

Secreted, Cell membrane.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human IgM from Human serum (Sigma#I8260).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP, FC, IF-Cell

Positive Control

Daudi cell lysate, human IgM protein, human tonsil tissue, human spleen tissue, Daudi.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200 ;FC: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 50 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes