NEDD8 Recombinant Rabbit Monoclonal Antibody [JF69-09]

About this product

NEDD8 is a ubiquitin (Ub)-like molecule that shares 80% homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation. NEDD8 and the corresponding yeast homolog Rub1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters. Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, Ubc12. NEDD8 preferentially associates with the CUL-2 protein in the E3 ligase complex CBCVHL, which consists of cullin proteins associating with elongin B/C and the tumor suppressor F-box protein, von Hippel Lindau (VHL). NEDD8 is predominantly localized to the nucleus and is highly expressed in adult heart and skeletal muscle. In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S Proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regulate proteolysis of intracellular proteins similar to other Ub-mediated pathways.

Specifications

Catalog number

ET1702-84

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

FLJ43224 antibody MGC104393 antibody MGC125896 antibody MGC125897 antibody NED8 antibody NEDD 8 antibody NEDD-8 antibody Nedd8 antibody NEDD8_HUMAN antibody Neddylin antibody Neural precursor cell expressed developmentally down regulated 8 antibody Neural precursor cell expressed developmentally down regulated gene 8 antibody Neural precursor cell expressed developmentally down-regulated protein 8 antibody Rub1 antibody Ubiquitin like protein Nedd 8 antibody Ubiquitin like protein Nedd8 antibody Ubiquitin-like protein Nedd8 antibody

Abbreviation

NEDD8

Swiss Prot

Q15843 Human, P29595 Mouse, Q71UE8 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthetic peptide within Human NEDD8 aa 12-57 / 81.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

COS-1 cell lysate, HeLa cell lysate, Raji cell lysate, HepG2 cell lysate, K-562 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, HepG2, human lung carcinoma tissue, human breast carcinoma tissue, human testis tissue, rat bladder tissue, mouse colon tissue, mouse heart tissue, mouse lung tissue, mouse skeletal muscle tissue, mouse spleen tissue, rat brain tissue, rat colon tissue, rat liver tissue, rat lung tissue, rat skeletal muscle tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:1,000 ;IP: 1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 9 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes