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Product details

Gli1 Recombinant Rabbit Monoclonal Antibody [JF09-08]

Catalog number ET1702-85

Discontinued

Price for ET1702-85

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Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Zinc finger protein GLI1 also known as glioma-associated oncogene is a protein that in humans is encoded by the GLI1 gene. It was originally isolated from human glioblastoma cells. The Gli proteins are the effectors of Hedgehog (Hh) signaling and have been shown to be involved in cell fate determination, proliferation and patterning in many cell types and most organs during embryo development. In the developing spinal cord the target genes of Gli proteins, that are themselves transcription factors, are arranged into a complex gene regulatory network that translates the extracellular concentration gradient of Sonic hedgehog into different cell fates along the dorsoventral axis. The Gli transcription factors activate/inhibit transcription by binding to Gli responsive genes and by interacting with the transcription complex. The Gli transcription factors have DNA binding zinc finger domains which bind to consensus sequences on their target genes to initiate or suppress transcription.

Specifications

Catalog number
ET1702-85
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
Yes
CAS Number
9000-83-3
Product Name Alternative
Gli 1 antibody GLI antibody GLI family zinc finger 1 antibody GLI Kruppel family member 1 antibody gli1 antibody GLI1_HUMAN antibody Glioma associated oncogene 1 antibody Glioma associated oncogene homolog 1 (zinc finger protein) antibody Glioma associated oncogene homolog antibody Glioma-associated oncogene antibody Oncogene GLI antibody Zfp 5 antibody Zfp5 antibody Zinc finger protein GLI 1 antibody Zinc finger protein GLI1 antibody
Abbreviation
GLI1
Swiss Prot
P08151 Human, P47806 Mouse,
Cellular Locus
Nucleus, Cytoplasm.
Host
Rabbit
Species Reactivity
Mouse, Rat, Human
Immunogen
Synthetic peptide within Human Gli1 aa 38-82 / 1106.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, IF-Cell
Positive Control
Rat testis tissue lysates, rat testis tissue, mouse fallopian tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:1,000 ;IHC-P:1:50-1:200 ;IF-Cell:1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 118 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Gli1 Recombinant Rabbit Monoclonal Antibody [JF09-08]?

The catalog number for Gli1 Recombinant Rabbit Monoclonal Antibody [JF09-08] is ET1702-85.

Is Gli1 Recombinant Rabbit Monoclonal Antibody [JF09-08] in stock?

Gli1 Recombinant Rabbit Monoclonal Antibody [JF09-08] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Gli1 Recombinant Rabbit Monoclonal Antibody [JF09-08] used for?

Zinc finger protein GLI1 also known as glioma-associated oncogene is a protein that in humans is encoded by the GLI1 gene. It was originally isolated from human glioblastoma cells. The Gli proteins are the effectors of Hedgehog (Hh) signaling and have been shown to be involved in cell fate determina

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

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