MiTF Recombinant Rabbit Monoclonal Antibody [JF100-01] — image 1 of 3

Product details

MiTF Recombinant Rabbit Monoclonal Antibody [JF100-01]

Catalog number ET1702-86

Discontinued

Price for ET1702-86

Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITF-deficient mice there is a complete absence of melanocytes.

Specifications

Catalog number: ET1702-86
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: Yes
CAS Number: 9000-83-3
Product Name Alternative: BHLHE32 antibody bHLHe32 antibody Class E basic helix-loop-helix protein 32 antibody CMM8 antibody Homolog of mouse microphthalmia antibody Mi antibody Microphthalmia associated transcription factor antibody Microphthalmia, mouse, homolog of antibody Microphthalmia-associated transcription factor antibody MITF antibody MITF_HUMAN antibody mitfa antibody nacre antibody WS2 antibody WS2A antibody z3A.1 antibody
Abbreviation: bHLHe32
Swiss Prot: O75030 Human, Q08874 Mouse, O88368 Rat
Cellular Locus: Nucleus, Cytoplasm.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein within Human MiTF aa 353-520 / 526.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, FC
Positive Control: SK-MEL-28 cell lysate, HeLa cell lysate, A375 cell lysate, A172 cell lysate, B16-F1 cell lysate, PC-12 cell lysate, Hela, A431, NIH/3T3, SW480.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000 ;IF-Cell: 1:50-1:100 ;FC: 1:50-1:100
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 59 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for MiTF Recombinant Rabbit Monoclonal Antibody [JF100-01]?

The catalog number for MiTF Recombinant Rabbit Monoclonal Antibody [JF100-01] is ET1702-86.

Is MiTF Recombinant Rabbit Monoclonal Antibody [JF100-01] in stock?

MiTF Recombinant Rabbit Monoclonal Antibody [JF100-01] is currently made to order / not held in immediate stock. Contact us for lead time.

What is MiTF Recombinant Rabbit Monoclonal Antibody [JF100-01] used for?

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.