alpha 1 Antitrypsin Recombinant Rabbit Monoclonal Antibody [JF10-03]

About this product

Alpha-1 antitrypsin or α1-antitrypsin (A1AT, α1AT, A1A, or AAT) is a protein belonging to the serpin superfamily. It is encoded in humans by the SERPINA1 gene. A protease inhibitor, it is also known as alpha1–proteinase inhibitor (A1PI) or alpha1-antiproteinase (A1AP) because it inhibits various proteases (not just trypsin). A1AT is a 52-kDa serpin and, in medicine, it is considered the most prominent serpin; the terms α1-antitrypsin and protease inhibitor (Pi) are often used interchangeably. Most serpins inactivate enzymes by binding to them covalently. These enzymes are released locally in relatively low concentrations where they are immediately cleared by proteins such as A1AT. Besides limiting elastase activity to limit tissue degradation, A1PI also acts to induce locomotion of lymphocytes through tissue including immature T cells through the thymus where immature T cells mature to become immunocompetent T cells that are released into tissue to elevate immune responsiveness. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis).

Specifications

Catalog number

ET1702-87

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

A1A antibody A1AT antibody A1AT_HUMAN antibody AAT antibody Alpha 1 antiproteinase antibody Alpha 1 antitrypsin antibody Alpha 1 antitrypsin null antibody Alpha 1 protease inhibitor antibody Alpha-1 protease inhibitor antibody Alpha-1-antiproteinase antibody alpha1 proteinase inhibitor antibody Alpha1AT antibody Dom1 antibody PI antibody PI1 antibody PRO2275 antibody Serine (or cysteine) proteinase inhibitor clade A member 1 antibody Serine protease inhibitor 1-1 antibody Serine protease inhibitor A1a antibody Serpin A1 antibody Serpin A1a antibody Serpin peptidase inhibitor clade A member 1 antibody Serpina1 antibody Short peptide from AAT antibody SPAAT antibody Spi1-1 antibody

Abbreviation

SERPINA1

Swiss Prot

P01009 Human

Cellular Locus

Secreted, Endoplasmic reticulum.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human alpha 1 Antitrypsin aa 1-418 / 418.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

Human spleen tissue lysates, MCF-7, Hela, HepG2, human spleen tissue, human liver tissue, human breast carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 47 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes