Myosin heavy chain Recombinant Rabbit Monoclonal Antibody [JF097-7]

About this product

Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain by myosin light chain kinase. Myosin heavy chains are encoded by the MYH gene family and have Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains, which were initially isolated from a human fetal skeletal muscle, are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chain are differentially expressed depending on the functional activity of the muscle.

Specifications

Catalog number

ET1702-88

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

cardiac muscle alpha isoform antibody MYH6 antibody MYH6_HUMAN antibody MYH7 antibody MYHC antibody MyHC-alpha antibody MyHC-beta antibody MYHCA antibody MYHCB antibody Myosin heavy chain 6 antibody Myosin heavy chain antibody Myosin heavy chain cardiac muscle alpha isoform antibody Myosin heavy chain cardiac muscle beta isoform antibody Myosin-6 antibody

Abbreviation

MyHC-alpha, MYH6

Swiss Prot

P13533 Human, P12883 Human, Q02566 Mouse, P02563 Rat

Cellular Locus

Cytoplasm, myofibril.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Myosin heavy chain aa 1220-1420 / 1939.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Tissue

Positive Control

Human skeletal muscle tissue lysates, rat skeletal muscle tissue, rat heart tissue, mouse smooth muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue lysates, human striated muscle tissue, human heart tissue, mouse heart tissue.

Concentration

1 mg/mL

Dilution

WB:1:500-1:2,000 ;IHC-P:1:100-1:500 ;IF-Tissue: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 224 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes