Ras Recombinant Rabbit Monoclonal Antibody [JF10-11]

About this product

The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family.

Specifications

Catalog number

ET1702-94

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

C-BAS/HAS antibody c-H-ras antibody C-HA-RAS1 antibody CTLO antibody GTPase HRas antibody GTPase KRas antibody GTPase NRas antibody H-Ras-1 antibody H-RASIDX antibody Ha-Ras antibody HAMSV antibody HRAS antibody HRAS1 antibody K RAS2A antibody K RAS2B antibody K RAS4A antibody K RAS4B antibody K-RAS antibody KRAS antibody KRAS1 antibody KRAS2 antibody N-RAS antibody N-terminally processed antibody NRAS antibody NRAS1 antibody p21ras antibody RASH_HUMAN antibody RASH1 antibody RASK2 antibody Transforming protein p21 antibody v Ha ras Harvey rat sarcoma viral oncogene homolog antibody v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody v ras neuroblastoma RAS viral oncogene homolog antibody

Abbreviation

NRAS, HRAS, KRAS

Swiss Prot

P01111 Human, P01112 Human, P01116 Human, P08556 Mouse, P32883 Mouse, Q61411 Mouse, P08644 Rat, P20171 Rat, Q04970 Rat

Cellular Locus

Cytoplasm, Cell membrane, Golgi apparatus, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human Ras aa 21-64 / 189.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IP, FC

Positive Control

MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, Mouse ovary tissue lysate, Rat brain tissue lysate, 293T cell lysate, Zebrafish tissue lysate, HeLa, PC-12.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100-1:500 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 21 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes