Dystrophin Recombinant Rabbit Monoclonal Antibody [JF1-022]

About this product

Dystrophin is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. This complex is variously known as the costamere or the dystrophin-associated protein complex (DAPC). Many muscle proteins, such as α-dystrobrevin, syncoilin, synemin, sarcoglycan, dystroglycan, and sarcospan, colocalize with dystrophin at the costamere. It has a molecular weight of 427 kDa. Dystrophin is coded for by the DMD gene – the largest known human gene, covering 2.4 megabases (0.08% of the human genome) at locus Xp21. The primary transcript in muscle measures about 2,100 kilobases and takes 16 hours to transcribe; the mature mRNA measures 14.0 kilobases. The 79-exon muscle transcript codes for a protein of 3685 amino acid residues. Spontaneous or inherited mutations in the dystrophin gene can cause different forms of muscular dystrophy, a disease characterized by progressive muscular wasting. The most common of these disorders caused by genetic defects in dystrophin is Duchenne muscular dystrophy.

Specifications

Catalog number

ET1702-98

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

BMD antibody CMD3B antibody DMD antibody DMD_HUMAN antibody Duchenne muscular dystrophy protein antibody Dystrophin antibody Muscular dystrophy Duchenne and Becker types antibody

Abbreviation

DMD

Swiss Prot

P11532 Human, P11531 Mouse, P11530 Rat

Cellular Locus

Cell membrane, Cytoplasm, Cell junction.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Dystrophin aa 3651-3685 / 3685.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IHC-Fr

Positive Control

Mouse skeletal muscle tissue lysate, Mouse heart tissue lysate, Rat skeletal muscle tissue lysate, Rat heart tissue lysate, human heart tissue, mouse heart tissue, rat heart tissue, human striated muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IHC-Fr: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 427 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes