C3 Recombinant Rabbit Monoclonal Antibody [JF10-30]

About this product

Complement C3 precursor contains complement C3 beta chain, complement C3 alpha chain, C3a anaphylatoxin, complement C3b alpha chain, complement C3c fragment, complement C3dg fragment, complement C3g fragment, complement C3d fragment and complement C3f fragment. C3a, C4a, and C5a are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage that aids in the elimination of pathogens. C3a and C5a secretion correlates with pathophysiological phenotypes such as asthma and bacterial meningitis. Binding of these proteins to their respective G protein-coupled receptors (C3aR, C5aR), which are present on the surface of myeloid leukocytes, induces proinflammatory events such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. C3aR is expressed in brain and activated B-lymphocytes whereas C5aR is prevalent on the surface of hepatocyte, lung, smooth muscle, and endothelial cells. Upon activation, C3aR and C5aR are susceptible to rapid GRK-mediated phosphorylation and clathrin-coated vesicle targeting. C5aR utilizes the Ras-Raf-ERK1/2 cascade and couples to Gi/G16 proteins.

Specifications

Catalog number

ET1702-99

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Acylation stimulating protein cleavage product antibody AHUS5 antibody ARMD9 antibody ASP antibody C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody C3 antibody CO3_HUMAN antibody Complement C3 antibody Complement C3c alpha' chain fragment 2 antibody Complement component 3 antibody Complement factor 3 antibody CPAMD1 antibody HEL S 62p antibody

Abbreviation

C3

Swiss Prot

P01024 Human, P01027 Mouse, P01026 Rat

Cellular Locus

Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human C3 aa 1,210-1,248 / 1,663.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, FC

Positive Control

Human liver tissue lysate, HepG2 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Mouse spleen tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, HepG2.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 187 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes