K63-linkage Specific Ubiquitin Recombinant Rabbit Monoclonal Antibody [JM09-67]

About this product

Ubiquitin is a small (8.6 kDa) regulatory protein found in most tissues of eukaryotic organisms. Four genes in the human genome code for ubiquitin: UBB, UBC, UBA52 and RPS27A. The addition of ubiquitin to a substrate protein is called ubiquitylation (or, alternatively, ubiquitination or ubiquitinylation). Ubiquitylation affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitylation involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. Only polyubiquitylation on defined lysines, mostly on K48 and K29, is related to degradation by the proteasome (referred to as the "molecular kiss of death"), while other polyubiquitylations (e.g. on K63, K11, K6 and M1) and monoubiquitylations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair.

Specifications

Catalog number

ET1703-07

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Epididymis secretory protein Li 50 antibody FLJ25987 antibody HEL S 50 antibody MGC8385 antibody Polyubiquitin B antibody RPS 27A antibody RPS27A antibody UBA 52 antibody UBA 80 antibody UBA52 antibody UBA80 antibody UBB antibody UBB_HUMAN antibody UBC antibody UBCEP 1 antibody UBCEP 2 antibody UBCEP1 antibody UBCEP2 antibody Ubiquitin antibody Ubiquitin B antibody

Abbreviation

UBB, UBC, RPS27A, UBA52

Swiss Prot

P0CG47 Human, P0CG48 Human, P62979 Human, P62987 Human, P0CG49 Mouse, P62991 Mouse, P0CG51 Rat, P62989 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human K63-linkage Specific Ubiquitin aa 36-85.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

Hela cell lysates, 293T, N2A, Hela, human liver cancer tissue, human colon cancer tissue, human spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15/18/26/77 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes