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Product details

HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10]

Catalog number ET1703-13

Price for ET1703-13

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Size / count 100 µL

About this product

A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 kDa) in size,[40] which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. In addition to functioning as a purification tag, GST acts as a chaperone for the attached protein, promoting its correct folding, as well as preventing it from becoming aggregated in inclusion bodies when expressed in bacteria. The GST tag can easily be removed following purification by addition of thrombin protease if a suitable cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids).

Specifications

Catalog number
ET1703-13
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
Glutathione S Transferase antibody Glutathione S-transferase class-mu 26 kDa isozyme antibody GST antibody
Host
Rabbit
Species Reactivity
Universal
Immunogen
recombinant protein
Isotype
IgG
Conjugation
HRP-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB
Positive Control
293T transfected with GST cell lysate, 293T transfected with GST-tagged Histone H3.1 cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:5,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10]?

The catalog number for HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10] is ET1703-13.

What sizes are available for HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10]?

HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10] is available in the following size(s): 100 µL.

Is HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10] in stock?

HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10] is currently made to order / not held in immediate stock. Contact us for lead time.

What is HRP Conjugated GST Recombinant Rabbit Monoclonal Antibody [JM57-10] used for?

A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 kDa) in size,[40] which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. In a

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.

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