Cyclophilin A Recombinant Rabbit Monoclonal Antibody [JM107-03]

About this product

The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication. Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes (9,10).

Specifications

Catalog number

ET1703-33

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cyclophilin A antibody Cyclophilin antibody CyclophilinA antibody Cyclosporin A binding protein antibody Cyclosporin A-binding protein antibody CYPA antibody CYPH antibody Epididymis secretory sperm binding protein Li 69p antibody HEL S 69p antibody MGC117158 antibody MGC12404 antibody MGC23397 antibody Peptidyl prolyl cis trans isomerase A antibody Peptidyl-prolyl cis-trans isomerase A antibody Peptidylprolyl isomerase A (cyclophilin A) antibody Peptidylprolyl isomerase A antibody PPIA antibody PPIA protein antibody PPIA_HUMAN antibody PPIase A antibody Rotamase A antibody RotamaseA antibody T cell cyclophilin antibody

Abbreviation

PPIase A, PPIA

Swiss Prot

P62937 Human, P17742 Mouse, P10111 Rat

Cellular Locus

Cytoplasm, Nucleus, Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Cyclophilin A aa 131-159 / 165.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

Hela cell lysate, A431 cell lysate, HepG2 cell lysate, human breast carcinoma tissue, human lung tissue, human pancreas tissue, mouse lung tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 18 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes