VAMP2 Recombinant Rabbit Monoclonal Antibody [JM11-00]

About this product

Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs, also designated synaptobrevins, including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including a- and γ-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exo-cytosis by competing with SNAP 25 and syntaxins for VAMP binding.

Specifications

Catalog number

ET1703-50

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

FLJ11460 antibody RATVAMPB antibody RATVAMPIR antibody SYB antibody SYB2 antibody Synaptobrevin 2 antibody Synaptobrevin-2 antibody VAMP 2 antibody VAMP-2 antibody Vamp2 antibody VAMP2_HUMAN antibody Vesicle associated membrane protein 2 antibody Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody Vesicle-associated membrane protein 2 antibody

Abbreviation

VAMP-2, VAMP2

Swiss Prot

P63027 Human, P63044 Mouse, P63045 Rat

Cellular Locus

Cell junction, Cell membrane, Cytoplasmic vesicle , Synapse, Synaptosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human VAMP2 aa 9-42 / 116.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, FC, IP, IHC-P

Positive Control

Jurkat cell lysates, N2A, SH-SY5Y, SHG-44, mouse brain tissue, rat brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration. ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 13 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes