NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20] — image 1 of 3

Product details

NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20]

Catalog number ET1703-57

Price for ET1703-57

Size / count 100 µL

About this product

Neurofilament-M (NF-M), for neurofilament medium polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease.

Specifications

Catalog number: ET1703-57
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: 150kDa medium antibody NEF3 antibody NEFM antibody Neurofilament 3 antibody NF160 antibody NFM antibody
Abbreviation: NF-M, NEFM
Swiss Prot: P07197 Human, P08553 Mouse, P12839 Rat
Cellular Locus: Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within Human NEFM aa 651-692 / 916.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Positive Control: 293T cell lysates, N2A, PC-12, SH-SY5Y, mouse brain tissue, rat brain tissue, human brain tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000-1:2,000;IF-Cell: 1:100-1:500;IF-Tissue: 1:100-1:500;IHC-P: 1:50-1:200;IP: Use at an assay dependent concentration.
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 102 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20]?

The catalog number for NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20] is ET1703-57.

What sizes are available for NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20]?

NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20] is available in the following size(s): 100 µL.

Is NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20] in stock?

NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20] is currently made to order / not held in immediate stock. Contact us for lead time.

What is NEFM Recombinant Rabbit Monoclonal Antibody [JM11-20] used for?

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.