TIA1 Recombinant Rabbit Monoclonal Antibody [JM42-11]

About this product

FAS, also referred to as CD95 or APO-1, is a type I transmembrane protein that plays a central role mediating viral immunity. TIA-1 and TIAR are two closely related proteins that possess three RRMs (RNA recognition motifs), designated RRM 1, 2 and 3. Although both TIA-1 and TIAR are thought to function as mediators of apoptotic cell death, their specific roles in such pathways are unknown. Unlike TIA-1, which is found in the granules of cytotoxic lymphocytes, TIAR expression is limited to the nucleus and found in a much broader range of cells including, but not limited to, cells of hematopoietic origin. TIAR is translocated to the cytoplasm shortly after FAS ligation and this event immediately proceeds the onset of DNA fragmentation. A novel serine/threonine kinase that is activated as a result of FAS ligation, designated FAST (FAS-activated serine/threonine), shows kinase specificity towards both TIA-1 and TIAR. In unstimulated Jurkat cells, FAST resides in the cytoplasm as a highly phosphorylated protein and is quickly dephosphorylated and activated in response to stimulated FAS.

Specifications

Catalog number

ET1703-59

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cytotoxic granule associated RNA binding protein 1 antibody Cytotoxic granule associated RNA binding protein antibody mTIA-1 antibody Nucleolysin TIA 1 isoform p40 antibody Nucleolysin TIA-1 isoform p40 antibody Nucleolysin TIA1 isoform p40 antibody p40 TIA 1 antibody p40-TIA-1 (containing p15-TIA-1) antibody p40-TIA-1 antibody RNA binding protein TIA 1 antibody RNA binding protein TIA1 antibody RNA-binding protein TIA-1 antibody T-cell-restricted intracellular antigen-1 antibody TIA 1 antibody TIA 1 cytotoxic granule associated RNA binding protein antibody Tia antibody TIA-1 antibody TIA1 antibody TIA1 cytotoxic granule associated RNA binding protein antibody TIA1 cytotoxic granule associated RNA binding protein like 1 antibody TIA1 protein antibody TIA1_HUMAN antibody TIAL1 antibody TIAR antibody WDM antibody

Abbreviation

TIA-1

Swiss Prot

P31483 Human, P52912 Mouse

Cellular Locus

Nucleus, Cytoplasm, Stress granule.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human TIA1 aa 353-386 / 386.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Tissue, IHC-P, IP

Positive Control

Jurkat cell lysate, K-562 cell lysate, MOLT-4 cell lysate, HepG2 cell lysate, A20 cell lysate, NIH/3T3 cell lysate, Human lung tissue lysate, human tonsil tissue, human spleen tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Tissue: 1:50 ;IHC-P: 1:200-1:1,000 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 43 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes