CD40L Recombinant Rabbit Monoclonal Antibody [JM11-34]

About this product

Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1.

Specifications

Catalog number

ET1703-64

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CD 40L antibody CD154 antibody CD40 antigen ligand antibody CD40 ligand antibody CD40 ligand, soluble form antibody CD40-L antibody CD40L antibody CD40L_HUMAN antibody CD40LG antibody gp39 antibody hCD40L antibody HIGM1 antibody IGM antibody IMD3 antibody T B cell activating molecule antibody T BAM antibody T-cell antigen Gp39 antibody TNF-related activation protein antibody TNFSF5 antibody TrAP antibody Tumor necrosis factor (ligand) superfamily member 5 antibody Tumor necrosis factor ligand superfamily member 5 antibody

Abbreviation

CD40-L, TRAP, CD40LG

Swiss Prot

P29965 Human, P27548 Mouse, Q9Z2V2 Rat

Cellular Locus

Cell membrane, Cell surface, Secreted.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human CD40L aa 103-150 / 261.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

Jurkat cell lysate, K-562 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat thymus tissue lysate, Hela, MCF-7, NIH/3T3, human spleen tissue, THP-1.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 29 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes