TDP43 Recombinant Rabbit Monoclonal Antibody [JM51-10]

About this product

TDP-43 is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. TDP-43 has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recent work has characterized the transcriptome-wide binding sites revealing that thousands of RNAs are bound by TDP-43 in neurons. TDP-43 was originally identified as a transcriptional repressor that binds to chromosomally integrated trans-activation response element (TAR) DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene and the apoA-II gene. In spinal motor neurons TDP-43 has also been shown in humans to be a low molecular weight neurofilament (hNFL) mRNA-binding protein. It has also shown to be a neuronal activity response factor in the dendrites of hippocampal neurons suggesting possible roles in regulating mRNA stability, transport and local translation in neurons. It has been demonstrated that zinc ions are able to induce aggregation of endogenous TDP-43 in cells. Moreover, zinc could bind to RNA binding domain of TDP-43 and induce the formation of amyloid-like aggregates in vitro.

Specifications

Catalog number

ET1703-74

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

ALS10 antibody OTTHUMP00000002171 antibody OTTHUMP00000002172 antibody OTTHUMP00000002173 antibody TADBP_HUMAN antibody TAR DNA binding protein 43 antibody TAR DNA binding protein antibody TAR DNA-binding protein 43 antibody TARDBP antibody TDP 43 antibody TDP-43 antibody TDP43 antibody

Abbreviation

TARDBP, TDP43

Swiss Prot

Q13148 Human, Q921F2 Mouse,

Cellular Locus

Cytoplasm, Mitochondrion, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human TDP43 aa 354-390 / 414; (2)aa 253-290 / 414.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IP, IF-Cell, IF-Tissue, IHC-P, FC, IHC-Fr

Positive Control

HeLa cell lysate, K-562 cell lysate, Neuro-2a cell lysate, mouse spleen tissue lysate, mouse brain tissue lysate, rat spleen tissue lysate, rat brain tissue lysate, HeLa, Neuro-2a, human spleen tissue, mouse placenta tissue, human pancreas tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, mouse cerebrum tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:5,000 ;IHC-Fr: 1:200-1:500 ;IF-Cell: 1:250-1:500 ;IF-Tissue: 1:100 ;IHC-P: 1:500 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 45 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes