Rac1-2-3 Recombinant Rabbit Monoclonal Antibody [JM11-29]

About this product

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.

Specifications

Catalog number

ET1703-80

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cell migration inducing gene 5 protein antibody EN 7 antibody OTTMUSP00000004488 antibody p21 Rac3 antibody p21-Rac3 antibody Rac1B antibody RAC3 antibody RAC3_HUMAN antibody RAS related C3 botulinum substrate 3 antibody Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) antibody Ras-related C3 botulinum toxin substrate 3 antibody Rho family small GTP binding protein Rac3 antibody RP23-84C12.18 antibody EN-7 antibody EN7 antibody GX antibody HSPC 022 antibody HSPC022 antibody p21 Rac 2 antibody p21 Rac2 antibody p21-Rac2 antibody p21Rac2 antibody RAC 2 antibody Rac2 antibody RAC2_HUMAN antibody Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 antibody Ras related C3 botulinum toxin substrate 2 antibody Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) antibody Ras related C3 botulinum toxin substrate 3 antibody Ras-related C3 botulinum toxin substrate 2 antibody Rho family small GTP binding protein Rac 2 antibody Rho family small GTP binding protein Rac2 antibody Small G protein antibody Cell migration-inducing gene 5 protein antibody MGC111543 antibody MIG5 antibody Migration inducing gene 5 antibody Migration inducing protein 5 antibody p21 Rac1 antibody p21-Rac1 antibody Rac 1 antibody RAC1 antibody RAC1_HUMAN antibody Ras like protein TC25 antibody Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody Ras-like protein TC25 antibody Ras-related C3 botulinum toxin substrate 1 antibody Rho family small GTP binding protein Rac1 antibody TC 25 antibody TC25 antibody

Abbreviation

p21-Rac1, p21-Rac2, p21-Rac3

Swiss Prot

P63000 Human, P15153 Human, P60763 Human, P63001 Mouse, Q6RUV5 Rat

Cellular Locus

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length protein of Human RAC1 aa 1-192 / 192.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P

Positive Control

MCF-7 cell lysate, PC-12 cell lysate, 293T, Hela, NIH/3T3, human spleen tissue, mouse brain tissue, rat brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:200 ;IHC-P: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 21 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes