CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55]

Catalog number ET1703-85

Discontinued

Price for ET1703-85

Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Specifications

Catalog number: ET1703-85
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: Yes
CAS Number: 9000-83-3
Product Name Alternative: Cas9 antibody CRISPR-associated endonuclease Cas9/Csn1 antibody CRISPR-Cas9/Csn1 antibody csn1 antibody SpyCas9 antibody
Abbreviation: cas9
Swiss Prot: Q99ZW2 StreptococcusPyogenes
Host: Rabbit
Species Reactivity: Streptococcus pyogenes
Immunogen: Recombinant protein within Streptococcus pyogenes cas9 aa 730-990 / 1368.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Positive Control: 293T transfected with Cas9 cell lysates.
Concentration: 1 mg/mL
Dilution: WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: 158 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55]?

The catalog number for CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] is ET1703-85.

Is CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] in stock?

CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] is currently made to order / not held in immediate stock. Contact us for lead time.

What is CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] used for?

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.