CTCF Recombinant Rabbit Monoclonal Antibody [JM10-61]

About this product

CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes. The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2 class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid beta-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development. CTCF is ubiquitously expressed and localized to the nucleus. During terminal differentiation, CTCF is negatively regulated by differential phosphorylation and also by decreases in CTCF mRNA and protein expression.

Specifications

Catalog number

ET1703-90

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

11 zinc finger protein antibody 11 zinc finger transcriptional repressor antibody 11-zinc finger protein antibody CCCTC binding factor (zinc finger protein) antibody CCCTC binding factor antibody CCCTC-binding factor antibody Ctcf antibody CTCF_HUMAN antibody CTCFL paralog antibody MRD21 antibody Transcriptional repressor CTCF antibody

Abbreviation

CTCF

Swiss Prot

P49711 Human, Q61164 Mouse, Q9R1D1 Rat

Cellular Locus

Nucleoplasm, Chromosome, centromere.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human CTCF aa 685-727 / 727.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, CUT&Tag-seq, ChIP

Positive Control

HeLa cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, zebrafish tissue lysates, hybrid fish (crucian-carp) brain tissue lysates, HeLa, MCF-7, NIH/3T3, human liver tissue, human kidney tissue, mouse colon tissue, human endometrium tissue, rat colon tissue, rat kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:2,000 ;FC: 1:500-1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 83 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes