53BP1 Recombinant Rabbit Monoclonal Antibody [JA66-11]

About this product

The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.

Specifications

Catalog number

ET1704-05

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

53 BP1 antibody 53BP1 antibody FLJ41424 antibody MGC138366 antibody p202 antibody p53 binding protein 1 antibody p53 BP1 antibody p53-binding protein 1 antibody p53BP1 antibody TP53 BP1 antibody TP53B_HUMAN antibody Tp53bp1 antibody TRP53 BP1 antibody Tumor protein 53 binding protein 1 antibody Tumor protein p53 binding protein 1 antibody Tumor suppressor p53 binding protein 1 antibody Tumor suppressor p53-binding protein 1 antibody

Abbreviation

53BP1, p53-binding protein 1, p53BP1, TP53BP1

Swiss Prot

Q12888 Human, P70399 Mouse,

Cellular Locus

Nucleus. Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human 53BP1 aa 1-50 / 1972.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

HeLa cell lysate, HT-29 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat heart tissue lysate, HeLa, human brain tissue, mouse spleen tissue, mouse testis tissue, rat testis tissue, human spleen tissue, human liver tissue, human cervix tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:200 ;IHC-P: 1:200 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 214 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes