PRKAR2B (regulatory subunit) Recombinant Rabbit Monoclonal Antibody [JA11-69]

About this product

The second messenger cyclic AMP mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. One of several regulatory subunits, p-PKA IIβ reg (cAMP-dependent protein kinase type II-beta regulatory subunit), also known as PRKAR2B, is a 418 amino acid protein that is phosphorylated by the activated catalytic chain. p-PKA IIβ reg knockout mice exhibit diminished white adipose tissue and were protected against diet-induced obesity and fatty livers,as well as markedly reduced leptin mRNA. Also playing a role in the immune response, p-PKA IIβ reg suppresses CREB transcriptional activity and down-regulates IL-2 production in T-lymphocytes.

Specifications

Catalog number

ET1704-15

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

AI451071 antibody AW061005 antibody cAMP dependent protein kinase type II beta regulatory chain antibody cAMP dependent protein kinase type II beta regulatory subunit antibody cAMP-dependent protein kinase type II-beta regulatory subunit antibody H RG363E19.2 antibody KAP3_HUMAN antibody MGC116401 antibody Pkarb2 antibody PRKAR 2 antibody PRKAR2 antibody PRKAR2B antibody Protein kinase cAMP dependent regulatory type II beta antibody RATDNA antibody RII beta antibody RII (beta) antibody RIIbeta antibody WUGSC:H RG363E19.2 antibody

Abbreviation

cAMP-dependent protein kinase type II-beta regulatory subunit, PRKAR2B

Swiss Prot

P31323 Human, P31324 Mouse, P12369 Rat

Cellular Locus

Cytoplasm. Cell membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human PRKAR2B aa 369-418 / 418.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IP, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

Rat brain tissue lysates, mouse brain tissue, rat brain tissue, Hela, N2A, SH-SY5Y.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes