MSH6 Recombinant Rabbit Monoclonal Antibody [JA84-26]

About this product

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.

Specifications

Catalog number

ET1704-30

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DNA mismatch repair protein Msh6 antibody G/T mismatch binding protein antibody G/T mismatch-binding protein antibody GTBP antibody GTMBP antibody hMSH6 antibody HNPCC 5 antibody HNPCC5 antibody HSAP antibody MSH 6 antibody MSH6 antibody MSH6_HUMAN antibody mutS (E. coli) homolog 6 antibody MutS alpha 160 kDa subunit antibody MutS homolog 6 (E. coli) antibody mutS homolog 6 antibody MutS-alpha 160 kDa subunit antibody p160 antibody Sperm associated protein antibody

Abbreviation

hMSH6, GTBP, GTMBP, p160, MSH6

Swiss Prot

P52701 Human, P54276 Mouse

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

recombinant protein within Human MSH6 aa 1250-1360 / 1360.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP

Positive Control

Hela, human tonsil tissue, mouse liver tissue, mouse colon tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000;IHC-P: 1:50-1:200;IP: 1:10-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

153 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes