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Product details

PDK1 Recombinant Rabbit Monoclonal Antibody [JA67-30]

Catalog number ET1704-66

Discontinued

Price for ET1704-66

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Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Mitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation and dephosphorylation leading to reactivation of PDH. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. PDK1 consists of alpha and beta subunits; the kinase activity resides in the alpha subunit. Three PDK isoenzymes have been identified in humans (PDK1, 2 and 3) and two have been identified in rodent (PDK1 and 2).

Specifications

Catalog number
ET1704-66
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
Yes
CAS Number
9000-83-3
Product Name Alternative
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody HGNC:8809 antibody Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody PDH kinase 1 antibody Pdk1 antibody PDK1_HUMAN antibody Pyruvate dehydrogenase kinase isoform 1 antibody Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
Abbreviation
PDH kinase 1, PDK1, PDHK1
Swiss Prot
Q15118 Human, Q8BFP9 Mouse, Q63065 Rat
Cellular Locus
Mitochondrion matrix.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within Human PDK1 aa 16-65 / 436.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, IP, FC, IF-Cell, IF-Tissue
Positive Control
Mouse heart tissue lysate, rat heart tissue lysate, Hela, NIH/3T3, mouse heart tissue, rat heart tissue.
Concentration
1 mg/mL
Dilution
WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:2,000 ;FC: 1:50-1:100 ;IF-Tissue: 1:200-1:400
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 49 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for PDK1 Recombinant Rabbit Monoclonal Antibody [JA67-30]?

The catalog number for PDK1 Recombinant Rabbit Monoclonal Antibody [JA67-30] is ET1704-66.

Is PDK1 Recombinant Rabbit Monoclonal Antibody [JA67-30] in stock?

PDK1 Recombinant Rabbit Monoclonal Antibody [JA67-30] is currently made to order / not held in immediate stock. Contact us for lead time.

What is PDK1 Recombinant Rabbit Monoclonal Antibody [JA67-30] used for?

Mitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

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