CDT1 Recombinant Rabbit Monoclonal Antibody [JA30-81]

About this product

Human Cdt1 is a nuclear localizing replication initiation factor that is expressed only during the G1 and S phases of the cell cycle. In conjunction with Cdc18, Cdt1 is required to load the MCM protein Cdc21 onto chromatin at the end of mitosis which is necessary to initiate DNA replication. After S-phase onset, Cdt1 protein levels decrease and are barely detectable in cells in early S-phase or G2. However, Cdt1 mRNA is expressed in S-phase-arrested cells, and its levels do not change dramatically during the cell cycle, suggesting that proteolytic degradation rather than transcriptional controls ensure proper accumulation of Cdt1. Cdt1 can associate with the DNA replication inhibitor geminin, which is present in the S and G2 phases of the cell cycle. Inhibition of DNA replication by geminin in cell-free DNA replication extracts can be reversed by the addition of excess Cdt1. Geminin may be responsible for preventing inappropriate origin firing by targeting Cdt1.

Specifications

Catalog number

ET1704-67

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CDT 1 antibody cdt1 antibody CDT1_HUMAN antibody Chromatin licensing and DNA replication factor 1 antibody DNA replication factor antibody DNA replication factor Cdt1 antibody Double parked antibody Double parked Drosophila homolog of antibody Double parked homolog antibody DUP antibody Retroviral integration site 1 antibody Retroviral integration site 2 antibody Retroviral integration site1 antibody Retroviral integration site2 antibody RIS 2 antibody RIS2 antibody

Abbreviation

DUP, CDT1

Swiss Prot

Q9H211 Human

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human CDT1 aa 497-546 / 546.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP

Positive Control

Hela cell lysate, 293 cell lysate, human skin tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:25-1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

60 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes