GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83] — image 1 of 3

Product details

GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83]

Catalog number ET1704-68

Price for ET1704-68

Size / count 100 µL

About this product

The family of stress-responsive protein kinases include HRI (heme-regulated inhibitor or EIF2AK1), PKR (EIF2AK2 or TIK), PERK (EIF2AK3) and GCN2 (EIF2AK4). These proteins phosphorylate the eukaryotic translation initiation factor 2α (eIF2α) on Ser 51 to regulate general and gene-specific protein synthesis. Phosphorylated eIF2α acts as an inhibitor of its guanine nucleotide exchange factor eIF2B. GCN2, a unique eIF2α kinase, exists in all eukaryotes from yeast to mammals. In mammals, expression of GCN2 is highest in liver and brain tissues. GCN2 primarily initiates the phosphorylation of eIF2α in response to UV, but has been shown to increase phosphorylation activity in response to serum starvation. Also, substitution of Asp 83 for Ala on eIF2α results in impaired phosphorylation by GCN2 and PKR, suggesting a contribution of remote residues to kinase-substrate recognition.

Specifications

Catalog number: ET1704-68
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: E2AK4_HUMAN antibody Eif2ak4 antibody Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody Eukaryotic translation initiation factor 2-alpha kinase 4 antibody GCN2 antibody GCN2 eIF2alpha kinase antibody GCN2 like protein antibody GCN2-like protein antibody KIAA1338 antibody MGCN2 antibody
Abbreviation: EIF2AK4, GCN2, KIAA1338
Swiss Prot: Q9P2K8 Human
Cellular Locus: Cytoplasm.
Host: Rabbit
Species Reactivity: Human
Immunogen: Synthetic peptide within Human GCN2 aa 1-50 / 1649.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: SKOV-3 cell lysates, A549, Hela, MCF-7, human tonsil tissue, human breast carcinoma tissue, human placenta tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 187 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83]?

The catalog number for GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83] is ET1704-68.

What sizes are available for GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83]?

GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83] is available in the following size(s): 100 µL.

Is GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83] in stock?

GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83] is currently made to order / not held in immediate stock. Contact us for lead time.

What is GCN2 Recombinant Rabbit Monoclonal Antibody [JA03-83] used for?

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.