IDH2 Recombinant Rabbit Monoclonal Antibody [JA55-31]

About this product

Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+)-dependent isozyme is a homodimer. The protein encoded by the IDH2 gene is the NADP (+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Somatic mosaic mutations of this gene have also been found associated to Ollier disease and Maffucci syndrome.

Specifications

Catalog number

ET1704-93

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

D2HGA2 antibody ICD-M antibody IDH antibody IDH2 antibody IDHM antibody IDHP_HUMAN antibody IDP antibody IDPM antibody Isocitrate dehydrogenase [NADP], mitochondrial antibody Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody mNADP-IDH antibody NADP (+)-specific ICDH antibody Oxalosuccinate decarboxylase antibody

Abbreviation

IDH, IDH2

Swiss Prot

P48735 Human, P54071 Mouse, P56574 Rat

Cellular Locus

Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human IDH2 aa 403-452 / 452.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, FC

Positive Control

SK-Br-3 cell lysate, THP-1 cell lysate, Jurkat cell lysate, mouse heart tissue lysate, human liver tissue, human tonsil tissue, human prostate tissue, rat heart tissue, mouse heart tissue, MCF-7.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 51 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes