Fascin Recombinant Rabbit Monoclonal Antibody [JM12-53]

About this product

Fascin is an actin bundling protein. Fascin binds beta-catenin,and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. Fascin localizes to actin-rich protrusions at the cell surface called filopodia. T regulatory cell adhesion to antigen presenting dendritic cell causes sequestration of Fascin-1, an actin-bundling protein essential for immunological synapse formation, and skews Fascin-1–dependent actin polarization in antigen presenting dendritic cells toward the T reg cell adhesion zone. Although it is reversible upon T regulatory cell disengagement, this sequestration of essential cytoskeletal components causes a lethargic state of dendritic cells, leading to reduced T cell priming. This suggests Treg-mediated suppression of antigen presenting cells is a multi-step process. In addition to CTLA-4 CD80/CD86 interaction fascin dependent polarization of cytoskeleton towards dendritic cell Treg immune synapse play a pivotal role. In normal tissue, inflammation and the immune response would be limited by secretion of TGF-β. TGF-β on the one hand induces fascin expression, but on the other hand, restricts activity of transcription factor NF-κB. This results to limited fascin expression and allows tissue to rebuild epithelial barriers. In cancer, instead, TGF-β does not restrict NF-κB activity, and both can increase fascin expression, disrupting tissue structure and function.

Specifications

Catalog number

ET1705-18

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

55 kDa actin bundling protein antibody 55 kDa actin-bundling protein antibody Actin bundling protein antibody actin bundling protein, 55-KD antibody FAN 1 antibody FAN1 antibody Fascin 1 antibody Fascin actin bundling protein 1 antibody Fascin antibody Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody Fascin homolog 1 antibody Fascin, sea urchin, homolog of, 1 antibody Fascin1 antibody FLJ38511 antibody FSCN 1 antibody FSCN1 antibody FSCN1_HUMAN antibody HSN antibody p55 antibody Singed (Drosophila) like (sea urchin fascin homolog like) antibody Singed drosophila homolog like antibody Singed like (fascin homolog sea urchin) (Drosophila) antibody Singed like (fascin homolog sea urchin) antibody Singed like protein antibody Singed, drosophila, homolog of antibody Singed-like protein antibody SNL antibody Strongylocentrotus purpuratus antibody

Abbreviation

FSCN1, FAN1, HSN, SNL

Swiss Prot

Q16658 Human, Q61553 Mouse, P85845 Rat

Cellular Locus

Cytoskeleton, stress fiber, cytosol, cell cortex, filopodium, invadopodium, microvillus, cell junction.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Fascin aa 221-389 / 493.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

HeLa cell lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, SH-SY5Y, human kidney tissue, rat brain tissue, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:2,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:1,000 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 54 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes