IKB epsilon Recombinant Rabbit Monoclonal Antibody [JM62-63]

About this product

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon, also known as NFKBIE, is a protein which in humans is encoded by the NFKBIE gene. NFKBIE protein expression is up-regulated following NF-κB activation and during myelopoiesis. NFKBIE is able to inhibit NF-κB-directed transactivation via cytoplasmic retention of REL proteins. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-κB transcription factor complex. The NF-κB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NF-κB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NF-κB interaction with other transcription factors, such as STAT (see STAT6), AP-1 (JUN), and NFAT (see NFATC1).

Specifications

Catalog number

ET1705-40

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

I kappa B epsilon antibody I-kappa-B-epsilon antibody IkappaBepsilon antibody IkB E antibody IkB-E antibody IkB-epsilon antibody IKBE antibody IKBE_HUMAN antibody MGC72568 antibody NF kappa B inhibitor epsilon antibody NF kappa BIE antibody NF-kappa-B inhibitor epsilon antibody NF-kappa-BIE antibody NFkappa BIE antibody NFkappaB inhibitor epsilon antibody NFKBIE antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon antibody OTTHUMP00000016522 antibody Slc35b2 antibody solute carrier family 35, member B2 antibody

Abbreviation

NF-kappa-BIE, IkB-E, IkB-epsilon, IkappaBepsilon

Swiss Prot

O00221 Human

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human IKB epsilon aa 175-224 / 500.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP

Positive Control

THP-1 cell lysate, Jurkat cell lysate, Hela cell lysate, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes