Id1 Recombinant Rabbit Monoclonal Antibody [JM92-13]

About this product

DNA-binding protein inhibitor ID-1 is a protein that in humans is encoded by the ID1 gene. The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. ID1 has been shown to interact weakly with MyoD but very tightly with ubiquitously expressed E proteins. E proteins heterodimerize with tissue restricted bHLH proteins such as Myod, NeuroD, etc. to form active transcription complexes so by sequestering E proteins, Id proteins can inhibit tissue restricted gene expression in multiple cell lineages using the same biochemical mechanism. Other interacting partners include CASK. ID1 can be used to mark endothelial progenitor cells which are critical to tumor growth and angiogenesis. Targeting ID1 results in decreased tumor growth. ID1 has been shown to be targeted by cannabidiol in certain gliomas and breast cancers.

Specifications

Catalog number

ET1705-49

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

bHLHb24 antibody Class B basic helix-loop-helix protein 24 antibody dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody DNA binding protein inhibitor ID 1 antibody DNA binding protein inhibitor ID1 antibody DNA-binding protein inhibitor ID-1 antibody Dominant negative helix loop helix protein antibody ID 1 antibody ID antibody ID1 antibody ID1_HUMAN antibody Inhibitor of Differentiation 1 antibody Inhibitor of DNA binding 1 antibody inhibitor of DNA binding 1, dominant negative helix-loop-helix protein antibody

Abbreviation

bHLHb24, ID1, BHLHB24, ID

Swiss Prot

P41134 Human

Cellular Locus

Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human Id1 aa 11-60 / 155.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

293T cell lysates, Hela cell lysate, HepG2 cell lysate, A549, Hela, HepG2, human tonsil tissue, human liver tissue, human colon tissue, human placenta tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 16 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes