Phospho-ATM (S1981) Recombinant Rabbit Monoclonal Antibody [JM93-25]

About this product

ATM serine/threonine kinase, symbol ATM, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Several of these targets, including p53, CHK2, BRCA1, NBS1 and H2AX are tumor suppressors. The ATM-mediated DNA damage response consists of a rapid and a delayed response. The protein kinase ATM may also be involved in mitochondrial homeostasis, as a regulator of mitochondrial autophagy (mitophagy) whereby old, dysfunctional mitochondria are removed. Increased ATM activity also occurs in viral infection where ATM is activated early during dengue virus infection as part of autophagy induction and ER stress response.

Specifications

Catalog number

ET1705-50

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

A-T mutated antibody A-T mutated homolog antibody AT mutated antibody AT1 antibody ATA antibody Ataxia telangiectasia mutated antibody Ataxia telangiectasia mutated gene antibody Ataxia telangiectasia mutated homolog (human) antibody Ataxia telangiectasia mutated homolog antibody ATC antibody ATD antibody ATDC antibody ATE antibody ATM antibody ATM serine/threonine kinase antibody ATM_HUMAN antibody DKFZp781A0353 antibody MGC74674 antibody OTTHUMP00000232981 antibody Serine protein kinase ATM antibody Serine-protein kinase ATM antibody Serine/threonine-protein kinase ATM antibody Tefu antibody TEL1 antibody TEL1, telomere maintenance 1, homolog antibody TELO1 antibody Telomere fusion protein antibody

Abbreviation

A-T mutated

Swiss Prot

Q13315 Human

Cellular Locus

Nucleus. Cytoplasmic vesicle.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser1981 of Human ATM aa 1,940-1,989 / 3,056.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

HeLa cell lysate, HeLa treated with 1μM Camptothecin for 1 hour cell lysate, HeLa treated with 20μM Etoposide for 2 hours cell lysate, HepG2 cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, HeLa cells treated with 1μM Camptothecin for 1 hour, human breast carcinoma tissue, human colon cancer tissue, HEK-293.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1,000 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 351 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes