Cyclophilin F Recombinant Rabbit Monoclonal Antibody [JM71-39]

About this product

Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of cyclosporin A (CsA) and is encoded by a single unique gene conserved between yeast to humans. Cyclophilin B (CyPB) is secreted in biological fluids such as blood or milk, and binds to a specific receptor present on human peripheral blood lymphocytes and expressed in Jurkat cells, a line of human lymphoblasts. Cyclophilin D (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Cyclophilin F (CyP3), is also known as PPIF, Cyp-D or Rotamase F, is a 207 amino acid mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore.

Specifications

Catalog number

ET1705-7

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cyclophilin 3 antibody cyclophilin D antibody Cyclophilin F antibody Cyp D antibody CyP M antibody CyP-D antibody CyP-M antibody CYP3 antibody CypD antibody hCyP3 antibody mitochondrial antibody Mitochondrial cyclophilin antibody Peptidyl prolyl cis trans isomerase mitochondral antibody Peptidyl-prolyl cis-trans isomerase F antibody Peptidyl-prolyl cis-trans isomerase F mitochondrial antibody Peptidylprolyl isomerase F (cyclophilin F) antibody Peptidylprolyl isomerase F antibody PPIase antibody PPIase F antibody Ppif antibody PPIF_HUMAN antibody Rotamase antibody Rotamase F antibody

Abbreviation

PPIase F, CyP-D, CypD, CyP-M, PPIF, CYP3

Swiss Prot

P30405 Human, Q99KR7 Mouse, P29117 Rat

Cellular Locus

Mitochondrion matrix.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Cyclophilin F aa 30-79 / 207.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP, FC

Positive Control

HEK-293 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human colon carcinoma tissue, human breast carcinoma tissue, mouse brain tissue, human colon tissue, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:400 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 22 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes