HuR / ELAVL1 Recombinant Rabbit Monoclonal Antibody [JM96-33]

About this product

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA.

Specifications

Catalog number

ET1705-81

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody ELAV like 1 antibody ELAV like RNA binding protein 1 antibody ELAV-like protein 1 antibody ELAV1 antibody ELAV1_HUMAN antibody Elavl1 antibody Embryonic lethal abnormal vision drosophila homolog like 1 antibody Hu Antigen R antibody Hu-antigen R antibody Hua antibody HuR antibody MelG antibody

Abbreviation

HuR, ELAVL1, HUR

Swiss Prot

Q15717 Human, P70372 Mouse, B5DF91 Rat

Cellular Locus

Cytoplasm. Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human HuR / ELAVL1 aa 50-99 / 326.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP, IF-Cell

Positive Control

A549 cell lysate, Rat spleen tissue lysate, Rat heart tisse lysate, PC-12 cell lysate, Mouse heart tissue lysate, Hela cell lysate, human cervix cancer tissue, mouse heart tissue, rat brain tissue, human colon cancer tissue, human breast tissue, mouse testis tissue, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:100-1:1,000 ;IP: 1-2μg/sample ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 36 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes