NRF1 Recombinant Rabbit Monoclonal Antibody [JM89-63]

About this product

The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.

Specifications

Catalog number

ET1705-86

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

alpha pal antibody alpha palindromic binding protein antibody Alpha palindromic-binding protein antibody Alpha-pal antibody locus control region factor 1 antibody NFE2 related factor 1 antibody NRF-1 antibody Nrf1 antibody NRF1_HUMAN antibody Nuclear respiratory factor 1 antibody

Abbreviation

NRF-1, Alpha-pal, NRF1

Swiss Prot

Q16656 Human, Q9WU00 Mouse, Q62792 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human NRF1 aa 333-382 / 503.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

HeLa cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, 293T, MCF7, human tonsil tissue, human thyroid tissue, mouse skeletal muscle tissue, mouse colon tissue, rat hippocampus tissue, rat brain tissue, 293T.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 54 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes