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Product details

Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43]

Catalog number ET1705-94

Price for ET1705-94

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Size / count 100 µL

About this product

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Specifications

Catalog number
ET1705-94
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
CMD1W antibody CMH15 antibody Epididymis luminal protein 114 antibody HEL114 antibody Metavinculin antibody MV antibody MVCL antibody OTTHUMP00000019861 antibody OTTHUMP00000019862 antibody VCL antibody VINC antibody VINC_HUMAN antibody Vinculin antibody
Abbreviation
MV, VCL
Swiss Prot
P18206 Human, Q64727 Mouse, P85972 Rat
Cellular Locus
Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within human Vinculin aa 1,000-1,060.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IF-Tissue, IHC-P
Positive Control
HepG2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse spleen tissue lysate, rat kidney tissue lysate, HeLa, human testis tissue.
Concentration
1 mg/mL
Dilution
WB: 1:5,000-1:20,000 ;IF-Cell: 1:200-1:1,000 ;IF-Tissue: 1:50-1:4,000 ;IHC-P: 1:200-1:20,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 124 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43]?

The catalog number for Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43] is ET1705-94.

What sizes are available for Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43]?

Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43] is available in the following size(s): 100 µL.

Is Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43] in stock?

Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43] used for?

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that a

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

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