Rad51 Recombinant Rabbit Monoclonal Antibody [JM54-26]

About this product

Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad52 can interact with Rad51. Rad54A of the DEAD-like helicase superfamily binds to double-strand DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer. MRE11 (meiotic recombination 11, ATLD, HNGS1) is a nuclear 3′-5′ exonuclease/endonuclease that associates with Rad50 and influences homologous recombination, telomere length maintenance, and DNA double-strand break repair. MRE11 is most abundant in proliferating tissues.

Specifications

Catalog number

ET1705-96

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

BRCA1/BRCA2 containing complex, subunit 5 antibody BRCC 5 antibody BRCC5 antibody DNA repair protein RAD51 homolog 1 antibody DNA repair protein rhp51 antibody FANCR antibody hRAD51 antibody HsRAD51 antibody HsT16930 antibody MRMV2 antibody Rad 51 antibody RAD51 antibody RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody RAD51 homolog A antibody RAD51 homolog antibody RAD51 recombinase antibody RAD51, S. cerevisiae, homolog of antibody RAD51_HUMAN antibody RAD51A antibody RECA antibody RecA like protein antibody RecA, E. coli, homolog of antibody Recombination protein A antibody

Abbreviation

HsRAD51, hRAD51, RAD51, RAD51A, RECA

Swiss Prot

Q06609 Human, Q08297 Mouse, B5DF04 Rat

Cellular Locus

Chromosome, Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Rad51 aa 1-50 / 339.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

Jurkat cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, U-2 OS, NIH/3T3, human ovary cancer tissue, human placenta tissue, human tonsil tissue, mouse spleen tissue, mouse testis tissue, rat spleen tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:100 ;IHC-P: 1:500-1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 37 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes