HPRT Recombinant Rabbit Monoclonal Antibody [JU03-26]

About this product

HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism.

Specifications

Catalog number

ET1706-08

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

HGPRT antibody HGPRTase antibody HPRT 1 antibody HPRT_HUMAN antibody HPRT1 antibody Hypoxanthine guanine phosphoribosyltransferase antibody Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody Hypoxanthine phosphoribosyltransferase 1 antibody Hypoxanthine-guanine phosphoribosyltransferase antibody

Abbreviation

HGPRT, HGPRTase, HPRT1, HPRT

Swiss Prot

P00492 Human, P00493 Mouse, P27605 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human HPRT aa 169-218 / 218.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IP

Positive Control

MCF-7 cell lysate, 293 cell lysate, Hela cell lysate, A431 cell lysate, rat kidney tissue lysate, rat tonsil brain lysate, mouse testis tissue lysate, mouse colon tissue lysate, zebrafish tissue lysates, rat brain tissue, human tonsil tissue, human colon carcinoma tissue, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

25 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes