IKBKE Recombinant Rabbit Monoclonal Antibody [JU06-72]

About this product

The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKα and IKKβ. IKK-i is primarily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFα, IL-1 and IL-6. Overexpression of IKK-i has been shown to result in phosphorylation of IκBα on Ser 32 and Ser 36, and in NFκB activation, suggesting that IKK-i may act as an IκB kinase in the immune system.

Specifications

Catalog number

ET1706-20

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

I kappa B kinase epsilon antibody I-kappa-B kinase epsilon antibody IkBKE antibody IKK related kinase epsilon antibody IKK-E antibody IKK-epsilon antibody IKK-i antibody IKKE antibody IKKE_HUMAN antibody IKKepsilon antibody IKKI antibody Inducible I kappa B kinase antibody Inducible I kappa-B kinase antibody Inducible IkappaB kinase antibody Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody KIAA0151 antibody MGC125294 antibody MGC125295 antibody MGC125297 antibody

Abbreviation

I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, IKK-i, IKBKE, IKKE, IKKI, KIAA0151

Swiss Prot

Q14164 Human

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human IKBKE aa 26-75 / 716.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, FC, IHC-P

Positive Control

Daudi cell lysate, Raji cell lysate, MCF-7 cell lysate, Jurkat cell lysate, Hela, JAR, SK-Br-3, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;FC: 1:50-1:100;IHC-P: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

80 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes