Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody [JU43-26]

About this product

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Specifications

Catalog number

ET1706-28

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

H3K14ac antibody

Abbreviation

HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ,H3K14ac

Swiss Prot

P68431 Human, P68433 Mouse, Q6LED0 Rat

Cellular Locus

Nucleus. Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Histone H3 aa 1-50 / 136 (acetyl K14) .

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, SNAP-ChIP, CUT&Tag-seq

Positive Control

Jurkat cell lysate, rat liver tissue lysate, mouse lung tissue lysate, Hela whole cell lysate, Hela treated with 1μM TSA for 18 hours whole cell lysate, F9, Hela, MCF-7, PC-3M, rat brain tissue, human kidney tissue, rat hippocampus tissue, human colon tissue, mouse testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:200 ;IHC-P: 1:500 ;SNAP-ChIP: 1:100 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

Yes